The aim of this project is to identify and quantify the chemical modifications in the bases and sugar of RNA in human cells. The RNA samples (mRNA, rRNA, tRNA) from different human cells will be prepared. The RNA samples along with RNA standards will be sent to three world-renowned labs in mass spectrometry. The three labs will analyze and determine the modifications. The modified bases and sugars identified by all three groups will be the consensus modifications. The goal of this project is to determine the modifications and their abundance in the RNA of human cells to set the stage of the Human RNome Project.
